http://www.freshpatents.com/Chemistry--molecular-biology-and-microbiology-dtnewntc435.php USPTO Class 435 - Chemistry: molecular biology and microbiology en-us Mon, 20 May 2013 13:41:55 -0700 1000 Wednesday Thursday Friday Saturday Sunday 141 31 http://www.freshpatents.com/index.php?rss=true http://www.freshpatents.com/images/freshpatentsnav_rss.gif http://www.freshpatents.com/-dt20130516ptan20130122483.php Mon, 20 May 2013 13:41:55 -0700 Described herein are kits for use in cryopreserving mammalian sperm including a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and monothioglycerol.... http://www.freshpatents.com/-dt20130516ptan20130122486.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a pharmaceutical composition comprising of preparations of human embryonic stem (hES) cells and their derivatives and methods for their transplantation into the human body, wherein transplantation results in the clinical reversal of symptoms, cure, stabilization or arrest of degeneration of a wide variety of presently... http://www.freshpatents.com/-dt20130516ptan20130122487.php Mon, 20 May 2013 13:41:55 -0700 Influenza viruses for use in preparing human vaccines have traditionally been grown on embryonated hen eggs, although more modern techniques grow the virus in mammalian cell culture e.g. on Vero, MDCK or PER.C6 cell lines. The inventor has realised that the conditions used for influenza virus culture can increase the... http://www.freshpatents.com/-dt20130516ptan20130122484.php Mon, 20 May 2013 13:41:55 -0700 The present specification relates to methods and kits for detection of animals that are persistently infected (PI) with a Bovine Viral Diarrhea Virus (BVDV) and/or transiently infected (TI) with BVDV. Some embodiments describe methods to distinguish a PI animal from a TI animal using a single one-time testing protocol.... http://www.freshpatents.com/-dt20130516ptan20130122485.php Mon, 20 May 2013 13:41:55 -0700 Provided are methods of analyzing biomaterials using a magnetic bead. The method may include preparing a bio material including a target material, preparing first and second magnetic beads, the second magnetic bead having a size smaller than that of the first magnetic bead, forming a binding element including the target... http://www.freshpatents.com/-dt20130516ptan20130122488.php Mon, 20 May 2013 13:41:55 -0700 There is provided an optical analysis technique enabling the detection of the condition or characteristic of a particle to be observed contained at a low concentration or number density in a sample solution using a light-emitting probe. The inventive optical analysis technique uses an optical system capable of detecting light... http://www.freshpatents.com/-dt20130516ptan20130122489.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base... http://www.freshpatents.com/-dt20130516ptan20130122490.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides labeled phospholink nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays, and others.... http://www.freshpatents.com/-dt20130516ptan20130122491.php Mon, 20 May 2013 13:41:55 -0700 There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided... http://www.freshpatents.com/-dt20130516ptan20130122500.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual.... http://www.freshpatents.com/-dt20130516ptan20130122494.php Mon, 20 May 2013 13:41:55 -0700 Assays for detecting and grading disease by assessing amounts of GSTP1 nucleic acid and ADAM protein in a sample, and methods of using the assays. In some embodiments, the assays use single molecule sequencing to simultaneously assay both GSTP1 nucleic acid and ADAM protein. The methods are especially useful for... http://www.freshpatents.com/-dt20130516ptan20130122493.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to methods, kits, probes, and systems for distinguishing between nucleotide variants that are close in proximity on a gene. The methods, kits, probes, and systems can include the use of a small amplicon assay in combination with two unlabeled probes in a high resolution thermal melting... http://www.freshpatents.com/-dt20130516ptan20130122492.php Mon, 20 May 2013 13:41:55 -0700 The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality... http://www.freshpatents.com/-dt20130516ptan20130122495.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a kit and nucleic acid chip for diagnosing bladder cancer using a bladder cancer-specific marker gene. More particularly, the invention relates to a kit and nucleic acid chip for diagnosing bladder cancer, which can detect the promoter methylation of a bladder cancer-specific gene, the promoter... http://www.freshpatents.com/-dt20130516ptan20130122503.php Mon, 20 May 2013 13:41:55 -0700 An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present... http://www.freshpatents.com/-dt20130516ptan20130122501.php Mon, 20 May 2013 13:41:55 -0700 Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.... http://www.freshpatents.com/-dt20130516ptan20130122502.php Mon, 20 May 2013 13:41:55 -0700 The disclosure provides probes for one or more target molecules. In particular examples, the probes include a molecular linker and first and second functional groups linked and spaced by the molecular linker, wherein the functional groups are capable of interacting with one another or with the target biomolecule in a... http://www.freshpatents.com/-dt20130516ptan20130122498.php Mon, 20 May 2013 13:41:55 -0700 This invention relates to novel nucleic acid-based probes and methods for detecting Plasmodium parasites in biological samples as well as detecting different Plasmodium parasites selectively from one another.... http://www.freshpatents.com/-dt20130516ptan20130122496.php Mon, 20 May 2013 13:41:55 -0700 Processes are disclosed for storing nucleic acid in a stable form. A solution comprising nucleic acid is applied to an unmodified, silica-based substrate whereby at least a portion of the nucleic acid binds to a surface of the substrate, the bound nucleic acid is washed and dried, and the resulting... http://www.freshpatents.com/-dt20130516ptan20130122499.php Mon, 20 May 2013 13:41:55 -0700 Systems and methods for measuring local copy number variation in DNA samples are provided. In particular, methods for detecting copy number variation in circulating free DNA (cfDNA) that may be used to assay for copy number variations often corresponding to cancerous cells or tumors are provided.... http://www.freshpatents.com/-dt20130516ptan20130122497.php Mon, 20 May 2013 13:41:55 -0700 This invention identifies and provides a recurrent translocation t(4;8) (p16.2; p23.1) associated with Adrenocortical Carcinoma, and diagnostic methods using the translocation by FISH hybridization or PCR based assays.... http://www.freshpatents.com/-dt20130516ptan20130122505.php Mon, 20 May 2013 13:41:55 -0700 The present teachings describe compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as a shiga toxin (stx) or an eae. In some embodiments, compositions, methods and kits can detect and identify... http://www.freshpatents.com/-dt20130516ptan20130122504.php Mon, 20 May 2013 13:41:55 -0700 Disclosed is determining expression levels of protective or harmful markers for bladder cancer prognosis; particularly, determining the expression level of COL4A3BP alone or in combination with expression levels of MBNL2, FABP4, and NEK1 or other markers where increased expression levels of these protective markers relative to a control correlates with... http://www.freshpatents.com/-dt20130516ptan20130122512.php Mon, 20 May 2013 13:41:55 -0700 An object of the present invention is to provide a method for determination or evaluation of an extract from inflamed tissues inoculated with vaccinia virus where the enhancement of activation of neurotrophic factor such as BDNF in cultured cells or the enhancement of activation of proteins participating therein is used... http://www.freshpatents.com/-dt20130516ptan20130122508.php Mon, 20 May 2013 13:41:55 -0700 Methods and devices for the thermal processing of samples are disclosed, including sample processing devices featuring an overflow region for retaining excess fluid, as well as portable sealing apparatuses for occluding channels in a sample processing device.... http://www.freshpatents.com/-dt20130516ptan20130122511.php Mon, 20 May 2013 13:41:55 -0700 Improved methods that increase the specificity and sensitivity of detection of small RNAs, including miRNAs, using oligonucleotide primers and nucleic acid amplification, are provided. Reaction conditions that result in preferential decrease in cDNA synthesis of RNAs other than the small RNA molecules targeted for detection during miRNA tailing and reverse... http://www.freshpatents.com/-dt20130516ptan20130122507.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides a method for amplification of a target nucleic acid sequence or signal, wherein an amplification reaction mixture is used which contains at least one reversibly modified oligonucleotide having a non-hydroxyl group 3′ end which can be converted into a hydroxyl 3′ end upon exposure to a... http://www.freshpatents.com/-dt20130516ptan20130122506.php Mon, 20 May 2013 13:41:55 -0700 fluorescent molecules linked via linkers). sugars, sugar chains, amino acids, and peptides; and one or more five-membered heterocyclic rings, one or more six-membered heterocyclic rings, and one or more fused heterocyclic rings, these heterocylic rings containing... http://www.freshpatents.com/-dt20130516ptan20130122509.php Mon, 20 May 2013 13:41:55 -0700 The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials... http://www.freshpatents.com/-dt20130516ptan20130122510.php Mon, 20 May 2013 13:41:55 -0700 The present invention is a biosensor apparatus that includes a substrate, a source on one side of the substrate, a drain spaced from the source, a conducting channel between the source and the drain, an insulator region, and receptors on a gate region for receiving target material. The receptors are... http://www.freshpatents.com/-dt20130516ptan20130122516.php Mon, 20 May 2013 13:41:55 -0700 Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags... http://www.freshpatents.com/-dt20130516ptan20130122513.php Mon, 20 May 2013 13:41:55 -0700 A sample acquiring device for detection of biological components in a liquid sample is disclosed, comprising a measurement cavity for receiving a liquid sample and a reagent comprising an antibody linked with a magnetic particle and arranged in a dry form inside the measurement cavity. A method is further disclosed,... http://www.freshpatents.com/-dt20130516ptan20130122515.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides an immunodiagnostic method for determining procalcitonin and procalcitonin derivatives in a biological sample of a patient, in particular in the monitoring and control of treatment and the monitoring of the progression of a local or systemic bacterial infection, inflammation, sepsis or neurodegenerative disease. In particular, the... http://www.freshpatents.com/-dt20130516ptan20130122517.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a method and kit for performing assays like immunoassays. The assays are performed by using two different types of magnetic beads.... http://www.freshpatents.com/-dt20130516ptan20130122514.php Mon, 20 May 2013 13:41:55 -0700 One aspect of the present disclosure encompasses methods for determining a protein kinase or phosphatase activity in a biological sample, comprising: contacting in a reaction mix a first test sample and a fluorescently-labeled peptide substrate capable of being modified by a protein phosphatase or a protein kinase, contacting the reaction... http://www.freshpatents.com/-dt20130516ptan20130122522.php Mon, 20 May 2013 13:41:55 -0700 This document provides methods and materials related to assessing mammals for vascular disease. This document also provides methods and materials related to assessing mammals for valvular disease. For example, methods and materials for assessing mammals for vascular disease using elevated levels of endothelial progenitor cells expressing a bone-related polypeptide (e.g.,... http://www.freshpatents.com/-dt20130516ptan20130122521.php Mon, 20 May 2013 13:41:55 -0700 Methods and compositions are provided for the differentiation and characterization of mammalian fibroblast from mesenchymal stem cells. The methods of the invention provide a means to obtain mesenchymal stem cell-derived fibroblast populations, e.g., seeded on a scaffold, which may be used in wound healing.... http://www.freshpatents.com/-dt20130516ptan20130122520.php Mon, 20 May 2013 13:41:55 -0700 The present disclosure relates to compositions and methods comprising cell surface markers for hES-derived cells, in particular, endoderm lineage cells including pancreatic endoderm-type cells, derived from hES cells.... http://www.freshpatents.com/-dt20130516ptan20130122523.php Mon, 20 May 2013 13:41:55 -0700 An in-vitro process for the quick determination of the infection status of a Mycobacterium tuberculosis infection from whole blood in terms of active or latent tuberculosis, comprising the steps of: stimulating an antigen-specific T cell that is present in a first sample of whole blood with purified protein derivative (PPD)... http://www.freshpatents.com/-dt20130516ptan20130122524.php Mon, 20 May 2013 13:41:55 -0700 This invention is directed to methods and compositions for diagnosis, prognosis and for determining methods of treatment. The physiological status of a cell present in a sample (e.g. clinical sample) can be used in diagnosis or prognosis of a condition (e.g. Chronic Lymphocytic Leukemia), in patient selection for therapy, to... http://www.freshpatents.com/-dt20130516ptan20130122525.php Mon, 20 May 2013 13:41:55 -0700 This disclosure is directed to methods of conducting dynamic single-molecule fluorescence studies such as sm-FRET on a membrane protein which permits observation and quantification of conformational dynamics of a membrane protein. Also disclosed herein are mutant membrane proteins in which one or more mutations have been introduced for affixing a... http://www.freshpatents.com/-dt20130516ptan20130122518.php Mon, 20 May 2013 13:41:55 -0700 A panel for monitoring levels of biomarkers, including an assay having at least one inflammation monitoring test, at least one oxidative stress monitoring test, and at least one antioxidant activity monitoring test. A method of monitoring an individual's health, by collecting a sample from the individual, applying the sample to... http://www.freshpatents.com/-dt20130516ptan20130122519.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to the identification and use of diagnostic markers for ischemic stroke of the lacunar subtype. The invention relates to devices and kits for performing these methods.... http://www.freshpatents.com/-dt20130516ptan20130122528.php Mon, 20 May 2013 13:41:55 -0700 The invention relates to methods, devices and systems for assessing appendicitis in a subject. More particularly, this invention relates to methods, devices and systems for assessing appendicitis in a subject by evaluating multiple biomarkers in a sample from the subject and comparing the values of the biomarker to a reference... http://www.freshpatents.com/-dt20130516ptan20130122526.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides a method for designing a re-targeted toxin conjugate for use in treating a medical condition or disease. Also provided, is the use of said conjugates in the manufacture of a medicament for treating medical conditions or diseases. The conjugates include a Targeting Moiety, which directs the... http://www.freshpatents.com/-dt20130516ptan20130122527.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects. In particular, the invention relates to methods and compositions selected to monitor cardiorenal syndrome using assays that detect NGAL, preferably together with assays that detect natriuretic peptides such as BNP. Such... http://www.freshpatents.com/-dt20130516ptan20130122529.php Mon, 20 May 2013 13:41:55 -0700 A method for diagnosing or predicting the risk of developing preeclampsia by measuring the level of A1 M and one or more of the compounds HbA, HbF in a sample and comparing the obtained values with reference values. By using a combination of information relating to A1 M and HbF... http://www.freshpatents.com/-dt20130516ptan20130122530.php Mon, 20 May 2013 13:41:55 -0700 Systems, kits, and methods for predicting the risk of an adverse event related to acute kidney injury AKI as a consequence of a surgical intervention in a subject. Embodiments of the system and methods include means and steps for determining an amount of liver-type fatty acid binding protein (L-FABP) in... http://www.freshpatents.com/-dt20130516ptan20130122532.php Mon, 20 May 2013 13:41:55 -0700 The present invention discloses a biological measuring device with auto coding capabilities. In accordance with one embodiment of the present invention, the biological measuring device with auto coding capabilities may include a test strip associated with a code pattern; and a code reader electrically coupled to the test strip to... http://www.freshpatents.com/-dt20130516ptan20130122531.php Mon, 20 May 2013 13:41:55 -0700 A nanostructured composite is provided which comprises a matrix comprising carbon nanofibers, and a biochemical receptor, especially an enzyme, immobilized in the matrix, where the carbon nanofibers have been subject to an oxidation treatment. In a preferred embodiment, the nanostructured composite further comprises a biomimetically synthesized silica matrix.... http://www.freshpatents.com/-dt20130516ptan20130122533.php Mon, 20 May 2013 13:41:55 -0700 A method is provided for preparing electrically conductive polymer and cellulose nanocomposite particles and nanocomposite materials. Cellulose microparticles coated with a conductive polymer are added to an acid solution for initiating an acid hydrolysis reaction for a prescribed time interval to form conductive polymer coated cellulose nanoparticles. After quenching the... http://www.freshpatents.com/-dt20130516ptan20130122536.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in... http://www.freshpatents.com/-dt20130516ptan20130122535.php Mon, 20 May 2013 13:41:55 -0700 Synthesis of many proteins is tightly controlled at the level of translation and plays an essential role in fundamental processes such as cell growth and proliferation, signaling, differentiation or death. Methods that allow imaging and identification of nascent proteins allow for dissecting regulation of translation, both spatially and temporally, including... http://www.freshpatents.com/-dt20130516ptan20130122534.php Mon, 20 May 2013 13:41:55 -0700 A process for carrying out hematoxylin and eosin staining in a microfluidic device is described.... http://www.freshpatents.com/-dt20130516ptan20130122538.php Mon, 20 May 2013 13:41:55 -0700 A method of diagnosing a pathological condition by detecting microparticles in a sample of bodily fluid using dynamic light scattering (DLS) is disclosed. The detection of microparticles in the bodily fluid by DLS may be used as an indicator of existing disease, to evaluate a risk of disease, as well,... http://www.freshpatents.com/-dt20130516ptan20130122537.php Mon, 20 May 2013 13:41:55 -0700 Optical sensors for use in examining and analyzing a fluid material are described. The optical sensors include a channel configured to transport a fluid material to be examined and analyzed, a first waveguide positioned adjacent to the channel and configured to guide radiation through the channel and a first tunable... http://www.freshpatents.com/-dt20130516ptan20130122539.php Mon, 20 May 2013 13:41:55 -0700 It is disclosed a microsieve comprising two layers, wherein the first layer is a membrane layer having a plurality of micropores contained therein and a thickness of about 10 μm to about 100 μm, and the second layer is a membrane support layer having a plurality of openings contained therein... http://www.freshpatents.com/-dt20130516ptan20130122540.php Mon, 20 May 2013 13:41:55 -0700 In the tissue pieces used as each tissue piece in a tissue array obtained by arranging the tissue pieces on a substrate in an array form, the method for forming a roll-shaped tissue piece 6 obtained by rolling a sheet-like tissue piece 8 is characterized by the facts that the... http://www.freshpatents.com/-dt20130516ptan20130122541.php Mon, 20 May 2013 13:41:55 -0700 This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes polyketides and 3-hydroxypropionic acid.... http://www.freshpatents.com/-dt20130516ptan20130122542.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to modified SAK gene having amino acid SEQ ID 2. The present invention further relates to process for cloning and expressing modified SAK gene fusion protein which imparts improved stability to the heterologous protein of interest. Further the invention relates to process of purification of recombinant... http://www.freshpatents.com/-dt20130516ptan20130122544.php Mon, 20 May 2013 13:41:55 -0700 DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe... http://www.freshpatents.com/-dt20130516ptan20130122543.php Mon, 20 May 2013 13:41:55 -0700 The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the... http://www.freshpatents.com/-dt20130516ptan20130122545.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.... http://www.freshpatents.com/-dt20130516ptan20130122548.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product... http://www.freshpatents.com/-dt20130516ptan20130122546.php Mon, 20 May 2013 13:41:55 -0700 provided that when the signal peptide originates from a natural precursor of a specific protein, the polypeptide of interest is different from the protein. X7 is Ala or Cys, X6 is Gln, Asn or His,... http://www.freshpatents.com/-dt20130516ptan20130122547.php Mon, 20 May 2013 13:41:55 -0700 A method of constructing a host for expression of an exogenous gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes of Schizosaccharomyces pombe such as psp3 (SPAC1006.01), sxa2 (SPAC1296.03c), ppp51 (SPAC22G7.01c) or ppp52 (SPBC18A7.01). An isolated or purified host cell in which a protease-associated... http://www.freshpatents.com/-dt20130516ptan20130122549.php Mon, 20 May 2013 13:41:55 -0700 This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is... http://www.freshpatents.com/-dt20130516ptan20130122552.php Mon, 20 May 2013 13:41:55 -0700 A sample block for use in the polymerase chain reaction, DNA sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block... http://www.freshpatents.com/-dt20130516ptan20130122550.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired... http://www.freshpatents.com/-dt20130516ptan20130122551.php Mon, 20 May 2013 13:41:55 -0700 Compositions and methods are provided for loop mediated isothermal amplification in which single stranded binding proteins are shown to protect primers from non-specific extension and to stimulate the rate of threshold amplification.... http://www.freshpatents.com/-dt20130516ptan20130122553.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can... http://www.freshpatents.com/-dt20130516ptan20130122555.php Mon, 20 May 2013 13:41:55 -0700 A saccharide-solution production method using a biomass material, including feeding a biomass material containing cellulose, hemicellulose, and lignin under a normal pressure to put it under an increased pressure; hydrothermally decomposing the biomass material using pressurized hot water by a hydrothermal decomposition unit; and dissolving a lignin component and a... http://www.freshpatents.com/-dt20130516ptan20130122554.php Mon, 20 May 2013 13:41:55 -0700 It is intended to provide a method for treating lignocellulosic biomass, which can reliably show the completion of the course by which pretreated lignocellulosic biomass is rendered flowable and thus transportable. The method for treating lignocellulosic biomass comprises a first saccharification step of saccharifying pretreated lignocellulosic biomass with stirring using... http://www.freshpatents.com/-dt20130516ptan20130122556.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides a novel process for producing a sulfur-containing α-amino acid compounds such as methionine. A process for producing a sulfur-containing α-amino acid compound (Compound 2) comprising a step of reacting a specific sulfur-containing amino alcohol compound (Compound 1) with the following transformants and others: <Transformants> (a) both... http://www.freshpatents.com/-dt20130516ptan20130122558.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the... http://www.freshpatents.com/-dt20130516ptan20130122557.php Mon, 20 May 2013 13:41:55 -0700 The invention is directed to isolated microorganisms, as well as biomasses, cultures, microbial oils, and compositions thereof. The invention also provides methods of producing the microbial oils and methods of using the isolated microorganisms, biomasses, and microbial oils.... http://www.freshpatents.com/-dt20130516ptan20130122559.php Mon, 20 May 2013 13:41:55 -0700 A method for synthesizing polyhydroxyalkanoate using a microorganism includes providing a Ralstonia eutropha strain; providing a fermentation tank and adding crude glycerol into the fermentation tank; adding a nitrogen source into the fermentation tank to adjust a carbon to nitrogen (C/N) ratio to 20:1 to 150:1; controlling temperature of the... http://www.freshpatents.com/-dt20130516ptan20130122560.php Mon, 20 May 2013 13:41:55 -0700 The present invention is directed to novel methods for production of 5-epi-β-vetivone, 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one and 2-isopropyl-6,10-dimethylspiro[4.5]deca-1,6-dien-8-one, which are useful for their fragrant qualities. Provided are methods for production of premnaspirodiene from a terpene substrate, methods for production of 5-epi-β-vetivone from premnaspirodiene as starting material, and methods for production of 2-isopropyl-6,10-dimethyl-spiro[4.5]deca-2,6-dien-8-one and... http://www.freshpatents.com/-dt20130516ptan20130122561.php Mon, 20 May 2013 13:41:55 -0700 This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allow recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation... http://www.freshpatents.com/-dt20130516ptan20130122563.php Mon, 20 May 2013 13:41:55 -0700 The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by... http://www.freshpatents.com/-dt20130516ptan20130122562.php Mon, 20 May 2013 13:41:55 -0700 This invention relates to a recombinant microorganism capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and one or more genes involved in isoprene... http://www.freshpatents.com/-dt20130516ptan20130122564.php Mon, 20 May 2013 13:41:55 -0700 A method of applying a nondestructive mechanical force on one or more cells in aqueous environment by inducing heat generated acoustic pressure pulses. The method comprises providing an energy transmission pattern to induce the applying of a desired nondestructive mechanical force on a at least one cell by forming a... http://www.freshpatents.com/-dt20130516ptan20130122565.php Mon, 20 May 2013 13:41:55 -0700 The present invention is directed to methods for inducing desired activity in enzymes or microorganisms capable of producing the enzymes. The invention is further directed to methods of stabilizing activity in microorganisms. In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase... http://www.freshpatents.com/-dt20130516ptan20130122566.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a protein having a β-galactoside-α2,3-sialyltransferase activity but substantially not having any neuraminidase activity, a nucleic acid encoding the enzyme protein, and a method of producing the enzyme using a microorganism transformed with a gene encoding the protein. The protein of the present invention is a... http://www.freshpatents.com/-dt20130516ptan20130122567.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides the field of enhancing proteins and in particular to that of proteins enhanced by molecular change. It provides a variant of a phytase that is termed enhanced in that is has better thermostability and/or activity than the original phytase. The invention also provides a nucleic acid... http://www.freshpatents.com/-dt20130516ptan20130122568.php Mon, 20 May 2013 13:41:55 -0700 Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction... http://www.freshpatents.com/-dt20130516ptan20130122569.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular... http://www.freshpatents.com/-dt20130516ptan20130122570.php Mon, 20 May 2013 13:41:55 -0700 The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.... http://www.freshpatents.com/-dt20130516ptan20130122571.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.... http://www.freshpatents.com/-dt20130516ptan20130122572.php Mon, 20 May 2013 13:41:55 -0700 Provided are methods, compositions, and kits for cloning of DNA using DNA topoisomerase. The methods comprise (I) combining into a mixture (A) a first polynucleotide comprising an origin of replication, a selectable marker, two topoisomerase recognition sequences, and two nicking agent recognition sequences, each of the topoisomerase recognition sequences being... http://www.freshpatents.com/-dt20130516ptan20130122573.php Mon, 20 May 2013 13:41:55 -0700 A strain of Francisella species wherein a gene which encodes for part of the glutamate metabolic pathway has been inactivated, and which is able to produce a protective immune response in an animal, for use as live prophylactic or therapeutic vaccine against infection by said Francisella species. Particularly effective strains... http://www.freshpatents.com/-dt20130516ptan20130122574.php Mon, 20 May 2013 13:41:55 -0700 The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps: a) incubating the sample with at least one protein-degrading compound; b) binding the target nucleic acid to a solid phase; c) eluting the target nucleic... http://www.freshpatents.com/-dt20130516ptan20130122575.php Mon, 20 May 2013 13:41:55 -0700 A device, system, and method to produce alcohol and Bio fuels from bulky organic matter with a Sea based Fermentor with low cost per unit volume, formed from a plastic separator barrier which is flexible plastic film, a solar based distillation still with vacuum assist are disclosed. In one embodiment,... http://www.freshpatents.com/-dt20130516ptan20130122576.php Mon, 20 May 2013 13:41:55 -0700 An apparatus for automatically analyzing a nucleic acid includes: a sample preprocessing device including a plurality of chambers in which reagents mixed with a sample are accommodated according to sample preprocessing process order for extracting a nucleic acid from the sample; and a nucleic amplifying and detecting device connected with... http://www.freshpatents.com/-dt20130516ptan20130122577.php Mon, 20 May 2013 13:41:55 -0700 Provided are kits for determining a nucleotide sequence of a target nucleic acid, the kit including at least one sequence specific binding protein and a detectable tag. In accordance with a kit for determining a nucleotide sequence of a target nucleic acid according to one exemplary embodiment and a method... http://www.freshpatents.com/-dt20130516ptan20130122578.php Mon, 20 May 2013 13:41:55 -0700 The cap is movable between a pre-testing configuration with the plunger outside of the testing container and a testing configuration in which the plunger is inside the testing container interior area. A gelatinous protein is situated in the interior area of the testing container and formulated to change color in... http://www.freshpatents.com/-dt20130516ptan20130122580.php Mon, 20 May 2013 13:41:55 -0700 The present invention provides a culture vessel for forming an aggregated cell mass which has at least two wells, wherein the wells are made from a light-shielding member and an inner surface of the wells is subjected to treatment to impart cellular non-adhesiveness.... http://www.freshpatents.com/-dt20130516ptan20130122579.php Mon, 20 May 2013 13:41:55 -0700 A fermenter for a biogas plant which operates with wet fermentation includes a fixed casing which surrounds a reaction space, an inlet for biomass and an outlet for the fermentation reside. A wall is arranged inside the casing, which separates the inlet from the outlet.... http://www.freshpatents.com/-dt20130516ptan20130122582.php Mon, 20 May 2013 13:41:55 -0700 A conventional shuttle vector constructed by fusing an E. coli-derived plasmid and a transformant-derived plasmid functions in both E. coli and the transformant bacterium, and there exists no expression vector that functions only in a non-E. coli transformant. The present invention provides an plasmid expression vector comprising (1) a plasmid... http://www.freshpatents.com/-dt20130516ptan20130122581.php Mon, 20 May 2013 13:41:55 -0700 Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; “TALENS”) are provided.... http://www.freshpatents.com/-dt20130516ptan20130122583.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a process for sterilizing implantable biomaterials. In particular, the invention relates to a process for sterilizing collagen-containing implantable biomaterials and storage thereafter.... http://www.freshpatents.com/-dt20130516ptan20130122584.php Mon, 20 May 2013 13:41:55 -0700 The present invention is directed to systems and methods of processing aspirated adipose tissue for the isolation of stromal vascular fraction derived stem cells.... http://www.freshpatents.com/-dt20130516ptan20130122585.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to synthetic gag and gagpol genes optimized for high level expression via codon optimization and the uses thereof for the efficient generation of vector particles. The invention further relates to the generation of packaging cells and vaccines based on the synthetic gag and gagpol genes.... http://www.freshpatents.com/-dt20130516ptan20130122586.php Mon, 20 May 2013 13:41:55 -0700 According to embodiments, a method of producing insulin-producing tissues (IPTs) by culturing comprises: preparing non-endocrinal epithelial cells (NEECs) and vascular endothelial cells (VECs), which have been isolated or originated from postnatal pancreata, preferably by capturing of NEECs by collagen; culturing in vitro the NEECs and the VECs at least partly... http://www.freshpatents.com/-dt20130516ptan20130122588.php Mon, 20 May 2013 13:41:55 -0700 The present invention relates to a pharmaceutical composition comprising of preparations of human embryonic stem (hES) cells and their derivatives and methods for their transplantation into the human body, wherein transplantation results in the clinical reversal of symptoms, cure, stabilization or arrest of degeneration of a wide variety of presently... http://www.freshpatents.com/-dt20130516ptan20130122587.php Mon, 20 May 2013 13:41:55 -0700 There is provided a method for differentiating human turbinate mesenchymal stromal cells into bone cells, cartilage cells, nerve cells, or fat cells, in which the method includes a step of culturing the mesenchymal stromal cells that are isolated from human inferior turbinate tissues in a medium containing growth factors; and... http://www.freshpatents.com/-dt20130516ptan20130122589.php Mon, 20 May 2013 13:41:55 -0700 The invention provides a method of producing a mesodermal lineage progenitor cell, by 10 application of one or more factors selected from the group consisting of activin, Wnt, BMP, FGF, an inhibitor of activin, GDF and NT to a culture of undifferentiated stem cells for a period of time sufficient... http://www.freshpatents.com/-dt20130516ptan20130122590.php Mon, 20 May 2013 13:41:55 -0700 The invention relates to kinase ligands and polyligands. In particular, the invention relates to ligands, homopolyligands, and heteropolyligands that modulate AKT activity. The ligands, homopolyligands, and heteropolyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands, homopolyligands, and heteropolyligands to a cellular localization signal,... http://www.freshpatents.com/-dt20130516ptan20130122591.php Mon, 20 May 2013 13:41:55 -0700 Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.... http://www.freshpatents.com/-dt20130516ptan20130122592.php Mon, 20 May 2013 13:41:55 -0700 Provided is a method for transferring an extraneous gene by an electroporation technique, which is applicable to a wide range of animal cells and is extremely remarkably improved in viability and gene transferring rate. Also provided is a method for transferring an extraneous gene by an electroporation technique with high...